Characterization of Three Pleiotropic Drug Resistance Transporter Genes and Their Participation in the Azole Resistance of Mucor circinelloides

Mucormycosis is a life-threatening opportunistic infection caused by certain members of the fungal order Mucorales. This associated with high mortality rate, which can reach nearly 100% depending on underlying condition patient. Treatment mucormycosis challenging because these fungi are intrinsically resistant to most routinely used antifungal agents, such as azoles. One possible mechanism azole resistance drug efflux catalyzed ATP binding cassette (ABC) transporter superfamily. The pleiotropic (PDR) subfamily ABC transporters closely resistance. genome Mucor circinelloides encodes eight putative PDR-type transporters. In this study, transcription pdr genes has been analyzed after treatment. Only pdr1 showed increased transcript level in response all tested Deletion gene susceptibility posaconazole, ravuconazole and isavuconazole altered growth ability mutant. deletion mutant, pdr2 pdr6 significantly increased. was also done create single double knock out mutants for three genes. After , mutant strains decreased, while resulted sensitivity against isavuconazole. Our result suggests that regulation interconnected participates fungus